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Career
* 1973-1978 study at the Faculty of Science, P. J. Šafárik University in Košice, Slovakia
* 1978-1979 PhD. study
* 1979-1985 research associate
* 1983 PhD. in Plant Physiology at Faculty of Natural Sciences, Comenius University in Bratislava
* 1985-1989 assistant Professor
* 1989 associate Professor of Genetics
* 1997-2003 vice-dean for International relations, Faculty of Science, P. J. Šafárik University, Košice
* 1998-2002 head of Department of Experimental Botany and Genetics
* since 2002head of the Department of Genetics
* 2002 professorship in Genetics at Comenius University in Bratislava
* since 2003 deputy vice-rector for international relations at P. J. Šafárik University in Košice
* 2009 Doctor of Science in Genetics at Comenius Univrsity in Bratislava
Awards
Dean's award for research results - 2003
Silver medal on the occasion of 45. anniversary of Faculty of Science, P. J. Šafárik University for distinguished contribution to development of Institute of Biology and Ecology and development of international relations - 2008
Rector's award for outstanding scientific results in Genetics - 2010
Gold medal on the occasion of 50. anniversary of foundation of Faculty of Science, P. J. Šafárik University in Košice
Study abroad
* 1979 Agrobotanical Institute in Tápiószele, Hungary
* 1981 Biological Research Centre in Szeged, Hungary
* 1985 IREX study trip at universities in Knoxville, Tennessee and Mineapolis-Saint Paul, Minnesota, USA
* 1990 University of Trondheim, Norway
* 1992 NATO Advanced Study Institute in Heraklion, Crete
Study Lectures
* Genetics
* Model Organisms in Genetics
* Evolutionary Biology
* Cytogenetics and Karyology
Research activities
Research team of Prof. Čellárová is involved in investigation of bioactive compounds with anticancer activity which are produced by some representatives of the genus Hypericum. Recently the study is mainly focused on comparative analysis of transcriptomic data with an aim to identify key genes and encoding enzymes of photodynamically active naphthodianthrones and phloroglucinols and their localization in the plant by the newest imaging technologies. Study of biosynthetic potential of endophytic microorganisms isolated de novo from some Hypericum species along with elicitation of secondary metabolism by biotic and abiotic factors represent as essential part of the study. The results will be used to design biotechnological alternative of their production.
Supervisor
* PhD. students in Genetics at present
RNDr. Anna Mišianiková
RNDr. Daniela Zubrická
RNDr. Odeta Czeranková
RNDr. Jana Henzelyová
Mgr. Miroslava Bálintová
Mgr. Zuzana Jurčacková
* Former PhD. students
Katarína Kimáková, PhD. (Associate Professor) Renáta Brutovská, PhD. Jana Halušková, PhD. Vanda Švehlíková, PhD. Patrícia Saxová, PhD. Katarína Bruňáková, PhD. Jana Koperdáková, PhD. Martina Urbanová, PhD. Ján Košuth, PhD. Zuzana Katkovčinová, PhD. Hedviga Košuthová Komarovská, PhD. Matúš Skyba, PhD. Linda Petijová, PhD.
* Former students who completed their PhD. study abroad
Eva Vranová, PhD. (University of Ghent, Belgium)
Zuzana Jasenčáková, PhD. (University of Halle, Germany)
Vladimír Wolf, PhD. (University of Bern, Switzerland)
Membership
* International Association for Plant Tissue Culture and Biotechnology (IAPTCB) - national correspondent for the Slovak Republic
* International Institute for Refrigeration (IIR) - member of C1 Commission for cryobiology and cryomedicine
* Genetic society of J.G.Mendel - member
Projects
Projects granted by Slovak agencies
* APVV SK-CN-2015-0026
Transcriptome analysis of Hypericum spp. for identification of candidate genes in biosynthesis of bioactive substances, 2016-2017, principal investigator
* APVV-14-0154
Transcriptome, metabolome and signalome of bioactive compounds with antitumour activities in the genus Hypericum, 2015 – 2018, principal investigator
* VEGA 1/0090/15
De novo RNA-seq transcriptome analysis aimed at identification of candidate genes coding for profiling secondary metabolites in the genus Hypericum. 2015-2018, principal investigator
* APVV-0040-10
Hypericin: biotechnology, signalome, photodynamic therapy. 2010-2013
* VEGA 1/0142/11
Hypericin and its potential role in plant defence mechanisms in the genus Hypericum, 2011-2015, principal investigator
* APVV-LPP-0021=09
Candidate genes in biosynthesis of hypericin and hyperforin in the genus Hypericum, 2009-2012, principal investigator
* APVV-0321-07
Hypericum spp. as a source of bioactive substances with anticancer properties. 2008-2010, principal investigator
* APVV-LPP-0015-07
Genes of secondary metabolism and cryogenic damage in the genus Hypericum spp. 2008-2012, principal investigator
* VEGA 1/0049/08
Genetic aspects of production of bioactive substances in the genus Hypericum. 2008-2010, principal investigator
* VEGA 1/2326/05
Study of expression of the hyp-1 gene encoding for key enzyme in biosynthetic pathway of hypericin. 2005-2007, principal investigator
* APVT-20-003704
Hypericum perforatum L.: Genetic aspects of hypericin synthesis and its phototoxic effects. 2005-2007, principal investigator
International projects
* COST-843 Quality Enhancement of Plant Production through Tissue Culture. 2000-2005, principal investigator
* COST-871 Cryopreservation of Crop Species in Europe (Cryoplanet). Management Committee member and principal investigator, 2006-2010
* Bio-production of active compounds from Hypericum species. Project of multilateral collaboration BMBF (Bundes Ministerium fur Bildung und Forschung). 2006-2007, principal investigator of the Slovak partner
* Evaluation of the impact of cryopreservation on the main physiological and genetic characteristics of in vitro regenerated plants. Project of bilateral collaboration Slovakia-Bulgaria. 2007-2008, principal investigator of the Slovak partner
* Structural and functional changes of Hypericum spp. tissues subjected to cryopreservation. Project of bilateral collaboration Slovakia-Bulgaria. 2011-2013, principal investigator of the Slovak partner
Academic projects
* Tempus UM_JEP 13050: Credit-based System at Faculties of Science in Slovakia. 1999-2001, co-ordinator
* EUA Quality Culture Round II: Doctoral Programme Project. 2004-2005, co-ordinator at P. J. Šafárik University
* EUA Quality Culture Round III: Women in Universities: Research, Teaching, Leadership. 2005-2006, co-ordinator at P. J. Šafárik University
* EUA Creativity Project. 2005-2006, co-ordinator at P. J. Šafárik University
Collaboration
* Comenius University, Bratislava, Slovakia
* Institute of Experimental Botany, Czech Academy of Sciences, Olomouc, Czech Republic
* Technical University of Dortmund, Germany
* Technical University in Copenhagen, Denmark
* University „La Sapienza“ in Rome, Italy
* Hypexplor Consortium www.hypexplor.com
* University of Sofia, Bulgaria
Selected papers 2009-2016
Soták M, Czeranková O, Klein D, Nigutová K, Altschmied L, Li L, Jose A, Wurtele ES, Čellárová E. Differentially Expressed Genes in Hypericin-Containing Hypericum perforatum Leaf Tissues as Revealed by De Novo Assembly of RNA-Seq. Plant Molecular Biology Reporter 2016, doi: 10.1007/s11105-016-0982-2, in press.
Mišianiková A, Zubrická D, Petijová L, Bruňáková K, Čellárová E.Effect of cryoprotectant solution and of cooling rate on crystallization temperature in cryopreserved Hypericum perforatum cell suspension cultures. Cryoletters 2016, in press .
Bruňáková K and Čellárová E.Conservation Strategies in the Genus Hypericum via Cryogenic Treatment. Front. Plant Sci. 2016, 7:558 doi: 10.3389/fpls.2016.00558, in press.
Kucharikova A, Kimakova K, Janfelt C, Čellarova E. Interspecific variation in localization of hypericins and phloroglucinols in the genus Hypericum as revealed by Desorption Electrospray Ionization Mass Spectrometry imaging. Physiologia plantarum. 2016, Vol. 157, s. 2-12.
Zubrická D., Mišianiková A, Henzelyová J., Valletta A., Angelis D., D’Auria F.D., Simonetti G., et al.: Xanthones from roots, hairy roots and cell suspension cultures of selected Hypericum species and their antifungal activity agains Candida albicans. Plant Cell Report. 2015, DOI 10.1007/s00299-015-1842-5
Kusari S., Sezgin S., Nigutová K., Čellárová E., Spiteller M.: Spatial chemo-profiling of hypericin and related phytochemicals in Hypericum species using MALDI-HRMS imaging. Anal Bioanal Chem. 2015, 407, pp. 4779-4791
Stoyanova-Koleva D., Stefanova M., Ganeva T.S., Čellárová E.: Structural modifications in the mesophyll associated with cryopreservation of seven Hypericum species. Biologia Plantarum. 2015, 59, 3, pp. 214-520
Bruňáková K., Petijová L., Zámečník J., Turečková V., Čellárová E.: The role of ABA in the freezing injury avoidance in two Hypericum species differing in frost tolerance and potential to synthesize hypericins. Plant Cell Tiss. Org. Cult., 2015, 122, pp. 45-56
Petijová L., Bruňáková K., Zámečník J., Zubrická D., Mišianiková A., Kimáková K., Čellárová E.: Relation between frost tolerance and post-cryogenic recovery in Hypericum spp., prijaté do tlače pre Cryoletters, 35, 2014, 171-179
Košuth J., Hrehorová D., Jaskolski M., Čellárová E.: Stress-induced expression and structure of the putative gene hyp-1 for hypericin biosynthesis. Plant Cell Tiss. Org. Cult. 114, 2013, 2007-2016
Stoyanova-Koleva D., Stefanova M., Čellárová E., Kapchina-Toteva V.: Chloroplast ultrastructure of Hypericum perforatum L. plants regenerated in vitro after cryopreservation. Biol. Plant. 57, 2013, 793-796
Skyba M., Petijová L., Košuth J., Petrova Koleva D., Gancheva Ganeva Ts., Kapchina-Toteva V., Čellárová E.: Oxidative stress and antioxidant response in Hypericum perforatum L. plants subjected to low temperature treatment. J. Plant Physiol. 169, 2012, 955-964
Petijová L., Skyba M., Čellárová E.: Genotype-dependent response of Hypericum perforatum L. shoot tips to cryogenic treatment: Effect of pre-culture conditions on post-thaw recovery. Plant Omics J. 5, 2012, 291-297
Kusari, S., Košuth, J., Čellárová, E., Spiteller, M.: Survival-strategies of endophytic Fusarium solani against indigenous camptothecin biosynthesis. Fungal Ecol. 4, 2011, 219-223
Košuth, J., Smelcerovic, A., Borsch, T., Zuehlke, S., Karppinen, K., Spiteller, M., Hohtola, A., Čellárová, E.: The hyp-1 gene is not limiting for hypericin biosynthesis in the genus Hypericum. Functional Plant Biol. 38, 2011, 35-43
Skyba M., Faltus, M., Zámečník, J., Čellárová, E.: Thermal analysis of cryopreserved Hypericum perforatum L. shoot tips: cooling regime dependent dehydration and ice growth. Thermochim. Acta 514, 2011, 22-27
Bruňáková K., Zámečník J., Urbanová M., Čellárová E.: Dehydration status of ABA-treated and cold-acclimated Hypericum perforatum L. shoot tips subjected to cryopreservation. Thermochim. Acta 525, 2011, 62-70
Skyba, M., Urbanová, M., Kapchina-Toteva, V., Košuth, J., Harding, K., Čellárová, E.: Physiological, biochemical and molecular characteristics of cryopreserved Hypericum perforatum l. shoots tips. Cryo Lett. 31, 2010, 249-260
Danova, K., Čellárová, E. Macková , A., Daxnerová, Z., Kapchina-Toteva, V.: In vitro culture of Hypericum rumeliacum Boiss. and production of phenolics and flavonoids. In vitro Cell. and Dev. Biol. – Plant. 46, 2010, 422-429
Komarovská, H., Košuth, J., Giovannini, A., Smelcerovic, A., Zuehlke, S., Čellárová, E.: Effect of number of rol genes integrations on phenotypic variation in hairy root-derived Hypericum perforatum L. plants. Z. Naturforsch. 65c, 2010, 701-712
Bruňáková, K., Košuth, J., Katkovčinová, Z., Lázárová, M., Čellárová, E.: Expression of two genes of paclitaxel biosynthetic pathway during germination of Taxus baccata L. zygotic embryos. Biol. Plant. 54, 2010, 515-519, 515-519
Miadoková, E, Chalupa, I., Vlčková, V., Ševčovičová, A., Kopasková, M., Hercegová A., Gašperová, P., Alfoldiová, Ľ., Komjatiová, M., Czanyiová, Z., Gálová, E., Čellárová, E., Vlček, D.: Genotoxicity and antigenotoxicity evaluation of non-photoactivated hypericin. Phytother. Res., 24, 2010, 90-95
Other
* chairperson for doctoral study in Genetics at P. J. Šafárik University
* member of the commission for doctoral study at Comenius University in Genetics and Genetoxicology
* member of the Scientific Grant Agency of the Slovak Republic (1999-2005, 2012-2016)
* Member of the Council of the State Programme for Science a Technology
* Member of the Scientific and Development Assistance Agency (2004-2005)
* Member of the Scientific and Innovation Assistance Agency (2006-2009)
* Member of the Scientific Board of the Faculty of Science, P. J. Šafárik University
* Member of the Scientific Board of P. J. Šafárik University (2003-2007)
* Institutional co-ordinator of the Socrates/Erasmus programme (2003-2007)
* Chair of the Board of Centres of Excellence at P. J. Šafárik University
* member of Accreditation Commission for evaluation of institutes of the Slovak Academy of Sciences
* member of the Commission for DSc defence in Genetics
* member of the Commission for DSc defence in Botany and Plant Physiolog
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