Career
* 1978 - 1983 Study at the Faculty of Science, P. J. Safarik University in Košice, Slovakia.
* 1983 - 1985 Research Associate in Laboratory of Veterinary Oncology, Institute of Experimental Veterinary Medicine, Košice, Slovakia.
* 1985 - 1997 Research Associate, Department of General Biology, Faculty of Science, Pavol Jozef Šafárik University in Košice.
* 1998 - 2002 Associate Professor of Biology and Head, Department of Celullar and Molecular Biology, Faculty of Science, Pavol Jozef Šafárik University in Košice.
* 2002 - present Head, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice.
* 2002 - present Acting Chief, Laboratory of Molecular Biology Diagnostics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice.
Awards
* Award of Society for Radiotherapy, Radiology and Radiation Physics of the Slovak Medicine Society for best paper in 1992.
* Faculty of Science Dean's Award for Research and Creative Achievements, 1999, 2003 and 2009.
Study
Lectures and seminars:
* Cytopathology
* Seminars in Cytopathology
* Experimental Hematology
* Seminars in Experimental Hematology
* Experimental Techniques in Biology
Teaching and training of students:
Former students (Ph.D.)
* RNDr. Mária Čekanová: "Mechanism of growth modulation of human lung cancer cells by pulmonary fibroblasts." Defence January 25, 2005, Faculty of Science, Pavol Jozef Šafárik University in Košice, Slovakia
* Ing. Viktor Horváth: "High effectiveness of platinum complexes with adamantylamine in overcoming resistance to cisplatin and suppressing proliferation of ovarian cancer cells in vitro." Defence June 22, 2006, Faculty of Science, Masaryk University in Brno, Czech Republic
* RNDr. Ján Kleban: „Modulation of hypericin-mediated photodynamic therapy by inhibitors of arachidonic acid metabolism in tumor cell experimental model.“ Defence December 5, 2007, Faculty of Science, P. J. Šafárik University in Košice, Slovakia
* RNDr. Jaromír Mikeš: „Sensitivity of normal versus tumour colon epithelial cells towards photodynamic therapy with hypericin and implication of p53 status in efficiency of this therapeutic approach.“ Defence September 23, 2008, Faculty of Science, P.J. Šafárik University in Košice, Slovakia
* RNDr. Lucia Grada Kuliková: „Different effects of single and fractionated light delivery regimes in photodynamic therapy with hypericin on HT-29 adenocarcinoma cells in vitro.“ Defence February 2, 2010, University of Naples Federico II, Naples, Italy
* RNDr. Ján Kovaľ: „The role of HER2 receptor and erythropoietin in modulation of hypericin-mediated photodynamic therapy.“ Defence February 24, 2010, Faculty of Science, P. J. Šafárik University in Košice, Slovakia
* RNDr. Rastislav Jendželovský: „Inhibition of transport systems and its impact on the outcome of photodynamic therapy in colon cancer cells.“ Defence February 24, 2010, Faculty of Science, P.J. Šafárik University in Košice, Slovakia
* RNDr. Martin Kello: "Modulation of photodynamic therapy by polyunsaturated fatty acids in experimental tumour model in vitro." Defence August 26, 2010, Faculty of Science, P. J. Šafárik University in Košice, Slovakia
* RNDr. Peter Ferenc: "Photodynamic therapy and modulation of signaling pathways by tyrosine kinase inhibitor genistein in cancer therapy." Defence August 26, 2010, Faculty of Science, P. J. Šafárik University in Košice, Slovakia
* RNDr. Miriam Bačkorová: "Cytotoxic effects of lichen secondary metabolites in experimental tumour model in vitro." Defence September 29, 2011, Faculty of Science, P. J. Šafárik University in Košice, Slovakia
Current students (Ph.D.):
* Mgr. Lucia Mikešová
* Mgr. Ján Kriška
* Mgr. Zuzana Papčová
* RNDr. Barbora Valeková
Former students (M.S.):
* Peter Solár, 1993
* Juraj Bacskai, 1994
* Tatiana Kundrátová, 1995
* Andrea Domonkošová, 1995
* Gabriela Nagyová, 1997
* Andrea Háziková, 1997
* Miriam Kučáková, 1998
* Lucia Slovinská, 1998
* Denisa Gálffyová, 1998
* Agáta Soroková, 1998
* Miriam Tomková, 1998
* Monika Valigová, 1998
* Janette Šalachová, 1999
* Zuzana Šándorčinová, 1999
* Jana Szepešiová, 1999
* Mária Šimková, 2000
* Edita Makovcová, 2001
* Zuzana Šutorová, 2003
Research activities
The research is focused on the interactions of phospho/lipids and their metabolism with specific drugs. The mechanisms of action of non-steroidal anti-inflammatory drugs (NSAIDs), which act as inhibitors of arachidonic acid metabolism pathways, catalyzed by cyclooxygenases, lipoxygenases and cytochrome P450 monooxygenases, are investigated in both normal and transformed cells of various origins. The role of (phospho)lipid metabolism, most notably polyunsaturated fatty acids in effects of anti-cancer drugs (hypericin, platinum, paclitaxel etc.) on a wide panel of cancer cell lines of different origin are being investigated. The signaling pathways involved in the control of cytokinetics are a primary subject of investigation. Particularly the role and balance at the level of a) regulatory proteins and receptors controlling apoptosis; b) molecules regulating cell cycle and proliferation, c) reactive oxygen species and lipid peroxides are to be evaluated.
Membership
* Chief, Section for Cell and Molecular Biology, Scientific Grant Agency of the Ministry of Education of the Slovak Republic and the Slovak Academy of Science;
* Member, Accreditation section for Life Science of the Ministry of Education of the Slovak Republic;
* Member, Scientific Board, Pavol Jozef Šafárik University in Košice, Slovakia;
* Member, Scientific Board, Faculty of Science, Pavol Jozef Šafárik University in Košice, Slovakia;
* Member, Scientific Board, Institute of Animal Physiology, Košice, Slovakia;
* Member, Scientific Board of five International Conferences (Cells I, II; Analytical Cytometry; Radiobiological Conference, International Radiobiological Conference);
* Member, Czech Society for Analytical Cytology;
* Member, Czecho-Slovak Biological Society.
Reviewer:
Served in the past and/or serving as a reviewer of many journals including the following (only selected recent journals are listed):
* Biochimica et Biophysica Acta – General Subjects
* Biochemical Pharmacology
* British Journal of Cancer
* Cancer Immunology Immunotherapy
* Current Cancer Drug Targets
* Cancer Letters
* Cancer Research
* Drugs
* Experimental Biology and Medicine
* European Journal of Pharmacology
* FEBS Letters
* International Immunopharmacology
* International Journal of Radiation Biology
* International Journal of Immunopharmacology
* Journal of Pharmacy and Pharmacology
* Journal of Photochemistry and Photobiology B: Biology
* Molecular and Cellular Biochemistry
* Physiological Research
* Radiation Research
* Radiation Measurements
* Toxicology Letters
Projects
* Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic and the Slovak Academy of Science (project VEGA MŠVVaŠ SR a SAV 1/0626/11): Project period: 1-1-2011 - 12-31-2014: "Non-caspase signaling pathways in cancer cell programmed cell death and impact of ABC transporters on cell resistance formation after hypericin?s photocytotoxic effect." Principal investigator: Dr. P. Fedoročko.
* Scientific Grant Agency of the Ministry of Education of the Slovak Republic and the Slovak Academy of Science (project VEGA MŠ SR a SAV 1/0296/09: Project period: 1-1-2009 - 12-31-2011: "Erythropoietin and its role in angiogenesis of ovarian adenocarcinoma cells." Deputy Head of project: Dr. P. Fedoročko.
* Slovak Research and Development Agency (project APVV-0040-10): Project period: 06-01-2011 - 10-31-2014. "Hypericin: biotechnology, signalome, photodynamic therapy." Head of project: Dr. P. Fedoročko.
* Slovak Research and Development Agency (project APVV-0001-07 „Centres for Research and Teaching Excellence"): Project period: 07-01-2008 - 12-31-2010. "Centre for Signalome Research." Head of project: Dr. P. Fedoročko.
* Slovak Research and Development Agency (project APVV-LPP-0062-09): Project period: 09-01-2009-08-31-2013. "Modulation of cell signalling pathways for targeted cancer therapy." Head of project: Dr. P. Fedoročko.
Collaboration
* Institute of Biophysics, Academy of Science of the Czech Republic, Brno;
* University of Naples Federico II, Department of Biologia e Patologia Cellulare e Molecolare, Naples, Italy;
* L. Pasteur Hospital, Košice;
* Tissue Bank of Burns Centre and Plastic Surgery, The First Private Hospital Ltd., Košice-Šaca;
* Gyncare, Ltd., Košice.
Recent publications
* Fedoročko P., Brezáni P., Macková O.: Radioprotection of mice by the bacterial extract Broncho-VaxomR: Haemopoietic stem cells and survival enhancement. Int.J.Radiat.Biol., 61, 511 518, 1992
* Fedoročko P., Brezáni P.: Radioprotection of mice by the bacterial extract Broncho Vaxom: Comparison of survival in five inbred mouse strains. Int.J.Immunotherapy, 8, 185 190, 1992
* Fedoročko P.: Liposomal muramyl tripeptide phosphatidylethanolamine (MTP-PE) promotes haemopoietic recovery in irradiated mouse. Int.J.Radiat.Biol., 65, 465-475, 1994
* Fedoročko P., Brezáni P., Macková N.O.: Radioprotective effects of WR-2721, Broncho-VaxomR, and their combinations: survival, myelopoietic restoration, and induction colony-stimulating activity in mice. Int.J. Immunopharmacol., 16, 177-184, 1994
* Fedoročko P., Macková N.O., Brezáni P., Kopka M.: Administration of the bacterial extract Broncho-VaxomR enhances radiation recovery and myelopoietic regeneration. Immunopharmacology, 28, 163-170, 1994
* Fedoročko P., Macková N.O.: Radioprotective effects of combination Broncho-Vaxom, a macrophage activator, and indomethacin, an inhibitor of prostaglandin production: Relationship to myelopoiesis. Eur.J.Haematol., 56, 54-61, 1996
* Fedoročko P., Domonkošová A., Kundrátová T., Macková N.O., Brezáni P., Fedoročková A.: Effects of cadmium on haemopoiesis in irradiated and non-irradiated mice: 1.Relationship to the number of myeloid progenitor cells. Physiol.Res., 45, 93-100, 1996
* Fedoročko P., Macková N.O.: Combined modality radioprotection: Enhancement of survival and hematopoietic recovery in gamma-irradiated mice by the joint use of liposomal muramyl tripeptide phosphatidylethanolamine (MTP-PE) and indomethacin. Int.J.Immunopharmacol., 18, 329-337, 1996
* Fedoročko P., Macková N.O.: Radioprotection of haemopoietic stem cells by a single injection of bacterial lysate – IRS-19 administered to mice before or after irradiation. Int.J. Immunopharmacol., 22, 989-999, 2000
* Fedoročko P., Egyed A., Vacek A.: Irradiation induces increased production of haemopoietic and proinflammatory cytokines in the mouse lung. Int.J.Radiat.Biol., 78:4, 305-313, 2002
* Hoferová Z., Fedoročko P., Hofmanová J., Hofer M., Znojil V., Minksová K., Souček K., Egyed A., Kozubík A.: The effect of nonsteroidal anti-inflammatory drugs ibuprofen, flurbiprofen, and diclofenac on in vitro and in vivo growth of mouse fibrosarcoma. Cancer Investigation, 20:4, 490-498, 2002
* Hoferová Z., Fedoročko P., Hofer M., Hofmanová J., Kozubík A.: Lipoxygenase inhibitors suppress proliferation of G5:113 fibrosarcoma cells in vitro but do not produce anti-fibrosarcoma activity in vivo. Neoplasma, 50:2, 102-109, 2003
* Fedoročko P., Hoferová Z., Hofer M., Brezáni P.: Administration of liposomal muramyl tripeptide phosphatidylethanolamine (MTP-PE) and diclofenac in the combination attenuates their anti-tumor activities. Neoplasma, 50:3, 176-184, 2003
* Hoferová Z., Souček K., Hofmanová J., Hofer M., Minksová K., Fedoročko P., Kozubík A.: In vitro proliferation of fibrosarcoma cells depends on intact functions of lipoxygenases and cytochrome P-450-monooxygenase. Cancer Investigation, 22:2, 234-247, 2004
* Horváth V., Blanářová O., Švihálková-Šindlerová L., Souček K., Hofmanová J., Sova P., Kroutil A., Fedoročko P., Kozubík A.: Platinum(IV) complex with adamantylamine overcomes intrinsic resistance to cisplatin in ovarian cancer cells. Gynecologic Oncology 102:1, 32-40, 2006
* Kleban J., Szilárdiová B., Mikeš J., Horváth V., Sačková V., Brezáni P., Hofmanová J., Kozubík A., Fedoročko P.: Pre-treatment of HT-29 cells with 5-LOX inhibitor (MK-886) induces changes in cell cycle and increases apoptosis after photodynamic therapy with hypericin. Journal of Photochemistry and Photobiology B: Biology 84:2, 79-88, 2006
* Cekanova M., Masi T., Plummer H.K. III, Majidi M., Fedorocko P., Schuller H.M.: Pulmonary fibroblasts stimulate the proliferation of cell lines from human lung adenocarcinomas. Anti-Cancer Drugs 17:7, 771-781, 2006
* Mikeš J., Kleban J., Sačková V., Horváth V., Jamborová E., Vaculová A., Kozubík A., Hofmanová J., Fedoročko P.: Necrosis predominates in the cell death of human colon adenocarcinoma HT-29 cells treated under variable conditions of photodynamic therapy with hypericin. Photochemical & Photobiological Sciences 6:7, 758-766, 2007
* Solár P., Horváth V., Kleban J., Kovaľ J., Solárová Z., Kozubík A., Fedoročko P.: Hsp90 inhibitor geldanamycin increases the sensitivity of resistant ovarian adenocarcinoma cell line A2780cis to cisplatin. Neoplasma, 54:2, 127-130, 2007
* Kožurková M., Sabolová D., Janovec L., Mikeš J., Kovaľ J., Ungvarský J., Štefanišinová M., Fedoročko P., Kristian P., Imrich J.: Cytotoxic activity of proflavine diureas: Synthesis, antitumor evaluation and DNA binding properties of 1',1''-(acridin-3,6-diyl)-3',3''-dialkyldiureas. Bioorganic & Medicinal Chemistry 16:7, 3976-3984, 2008
* Dvorožňáková E., Porubcová J., Šnábel V., Fedoročko P.: Imunomodulative effect of liposomized muramyltripeptide phosphatidylethyanolamine (L-MTP-PE) on mice with alveolar echinococcosis and treated with albendazole. Parasitology Research 103:4, 919-929, 2008
* Solár P., Kovaľ J., Kleban J., Solárová Z., Lazúr J., Fedoročko P., Sytkowski A.J.: Erythropoietin inhibits apoptosis induced by photodynamic therapy in SKOV3 cells. Molecular Cancer Therapeutics 7:8, 2263-2271, 2008
* Mikeš J., Kovaľ J., Jendželovský R., Sačková V., Uhrinová I., Kello M., Kuliková L., Fedoročko P.: The role of p53 in efficiency photodynamic therapy with hypericin and subsequent long-term survival of colon cancer cells. Photochemical & Photobiological Sciences, 8:11, 1558-1567, 2009
* Jendželovský R., Mikeš J., Kovaľ J., Souček K., Procházková J., Kello M., Sačková V., Hofmanová J., Kozubík A., Fedoročko P.: Inhibition of MRP1 and BCRP activity by proadifen increases hypericin content and potentiates the effect of hypericin-mediated photodynamic therapy in HT-29 colon adenocarcinoma cells. Photochemical & Photobiological Sciences, 8:12, 1716-1723, 2009
* Ferenc P., Solár P., Kleban J., Mikeš J., Fedoročko P.: Down-regulation of Bcl-2 and Akt induced by combination of photoactivated hypericin and genistein in human breast cancer cells. Journal of Photochemistry and Photobiology B: Biology 98:1, 25-34, 2010
* Kovaľ J., Mikeš J., Jendželovský R., Kello M., Solár P., Fedoročko P.: Degradation of HER2 receptor through hypericin-mediated photodynamic therapy. Photochemistry and Photobiology, 86:1, 200-205, 2010
* Kello M., Mikeš J., Jendželovský R., Kovaľ J., Fedoročko P.: PUFAs enhance oxidative stress and apoptosis in tumour cells exposed to hypericin-mediated PDT. Photochemical & Photobiological Sciences 9:9, 1244-1251, 2010
* Kuliková L., Mikeš J., Hýžďalová M., Palumbo G., Fedoročko P.: NF-É»B is not directly responsible for photo-resistance induced by fractionated light delivery in HT-29 colon adenocarcinoma cells. Photochemistry and Photobiology 86: 1285-1293, 2010
* Solár P., Ferenc P., Solárová Z., Kovaľ J., Hrčková G., Fulton B.L., Fedoročko P.: Photoactivated hypericin induces downregulation of HER2 gene expression. Radiation Research 175: 51-56, 2011
* Bačkorová M., Bačkor M., Mikeš J., Jendželovský R., Fedoročko P.: Variable responses of different human cancer cells to the lichen compounds parietin, atranorin, usnic acid and gyrophoric acid. Toxicology in Vitro 25: 37-44, 2011
* Mikeš J., Hýžďalová M., Kočí L., Jendželovský R., Kovaľ J., Vaculová A., Hofmanová J., Kozubík A., Fedoročko P.: Lower sensitivity of FHC fetal colon epithelial cells to photodynamic therapy compared to HT-29 colon adenocarcinoma cells despite higher intracellular accumulation of hypericin. Photochemical & Photobiological Sciences, 10: 626-632, 2011
* Ferenc P., Solár P., Mikeš J., Kovaľ J., Fedoročko P.: Breast Cancer and Current Therapeutic Approaches: From Radiation to Photodynamic Therapy, Breast Cancer - Current and Alternative Therapeutic Modalities, Esra Gunduz and Mehmet Gunduz (Ed.), pp. 63-88, 2011, ISBN: 978-953-307-776-5, InTech, Open Access Publisher, Available from: http://www.intechopen.com/articles/show/title/breast-cancer-and-current-therapeutic-approaches-from-radiation-to-photodynamic-therapy
* Kovaľ J., Solár P., Mikeš J., Jendželovský R., Fedoročko P.: Targeting HER2 with Photodynamic Therapy. In: HER2 and Cancer: Mechanism, Testing and Targeted Therapy (Eds.: Sophie I. Williams and Christopher E. Rogers), Nova Science Publishers, Inc., ISBN: 978-1-61122-650-8, pp. 1-46, 2011
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